Share Email Print
cover

Proceedings Paper

Confocal microscope with enhanced depth discrimination
Author(s): Roland V. Shack; Peter H. Bartels; Donald W. Hillman; M. Sabb; Richard L. Shoemaker
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Confocal microscopy, in comparison to conventional microscopy, offers a narrower point-spread function, the rendition of phase structures in contrast, and high axial resolution. Of these, the capabilities of optical sectioning and the three-dimensional imaging of cells and tissues have attracted the most interest. The literature offers a number of studies exploring the factors affecting depth of field in confocal microscopy. In this article, the three-dimensional representation of fluorescence imagery is examined and the design of a laser scanning confocal fluorescence microscope with enhanced depth discrimination is described.

Paper Details

Date Published: 1 July 1990
PDF: 7 pages
Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); doi: 10.1117/12.17804
Show Author Affiliations
Roland V. Shack, Optical Sciences Ctr./Univ. of Arizona (United States)
Peter H. Bartels, Optical Sciences Ctr./Univ. of Arizona (United States)
Donald W. Hillman, Optical Sciences Ctr./Univ. of Arizona (United States)
M. Sabb, Optical Sciences Ctr./Univ. of Arizona (United States)
Richard L. Shoemaker, Optical Sciences Ctr./Univ. of Arizona (United States)


Published in SPIE Proceedings Vol. 1206:
New Technologies in Cytometry and Molecular Biology
Gary C. Salzman, Editor(s)

© SPIE. Terms of Use
Back to Top