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Proceedings Paper

Confocal bioimaging the living cornea with autofluorescence and specific fluorescent probes
Author(s): Barry R. Masters; Stephen W. Paddock
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Paper Abstract

Confocal bioimaging of the fine structure of the living rabbit cornea with both reflected light and fluorescent light has been demonstrated with a laser scanning confocal imaging system. Kalman averaging was used to reduce the noise in the images. Superficial epithelial, basal epithelial cells, stromal keratocytes, and endothelial cells were imaged. These cells and their subcellular structures were imaged in the two modes for comparison. The superficial epithelial cells were imaged by their autofluorescence (488/520 nm). This fluorescence signal may be due to the mitochondrial flavoproteins and can be used as a noninvasive indicator of cellular oxidative function. Thiazole orange was used to stain cell nuclei for fluorescence imaging. DiOC6 was used to stain the endoplasmic reticulum for fluorescence imaging. Fluorescein- conjugated phalloidin was used to stain actin for fluorescence imaging.

Paper Details

Date Published: 1 August 1990
PDF: 15 pages
Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); doi: 10.1117/12.17793
Show Author Affiliations
Barry R. Masters, Georgia Institute of Technology (United States)
Stephen W. Paddock, Univ. of Wisconsin/Madison (United States)


Published in SPIE Proceedings Vol. 1205:
Bioimaging and Two-Dimensional Spectroscopy
Louis C. Smith, Editor(s)

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