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Proceedings Paper

Three-dimensional organization of chromosomes studied by in-situ hybridization and optical-sectioning microscopy
Author(s): Yasushi Hiraoki; Mary C. Rykowski; Jeffrey A. Lefstin; David A. Agard; John W. Sedat
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Paper Abstract

We have examined the three-dimensional arrangement of chromosomes in embryos of Drosophila melanogaster at the syncytial blastoderm stage using three-dimensional optical sectioning microscopy. High-resolution optical sectioning in fixed embryos, in conjunction with in situ hybridization, has revealed the location of specific chromosomal regions within diploid interphase nuclei, as well as the spatial arrangement of mitotic chromosomes. Time-lapse in vivo optical sectioning has revealed the dynamic behavior of chromosomes throughout the mitotic cycle. Combination of these observations has provided insights into the dynamic aspects of three-dimensional chromosome behavior.

Paper Details

Date Published: 1 August 1990
PDF: 9 pages
Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); doi: 10.1117/12.17779
Show Author Affiliations
Yasushi Hiraoki, Univ. of California/San Francisco (United States)
Mary C. Rykowski, Univ. of California/San Francisco (United States)
Jeffrey A. Lefstin, Univ. of California/San Francisco (United States)
David A. Agard, Univ. of California/San Francisco (United States)
John W. Sedat, Univ. of California/San Francisco (United States)

Published in SPIE Proceedings Vol. 1205:
Bioimaging and Two-Dimensional Spectroscopy
Louis C. Smith, Editor(s)

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