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Proceedings Paper

Fluorescence decay time imaging using an imaging photon detector with a radio frequency photon correlation system
Author(s): Christopher G. Morgan; A. C. Mitchell; J. G. Murray
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Paper Abstract

An imaging photon detector has been modified to incorporate fast timing electronics coupled to a custom built photon correlator interfaced to a RISC computer. Using excitation with intensity- muodulated light, fluorescence images can be readily obtained where contrast is determined by the decay time of emission, rather than by intensity. This technology is readily extended to multifrequency phase/demodulation fluorescence imaging or to differential polarised phase fluorometry. The potential use of the correlator for confocal imaging with a laser scanner is also briefly discussed.

Paper Details

Date Published: 1 May 1990
PDF: 10 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17773
Show Author Affiliations
Christopher G. Morgan, Univ. of Salford (United Kingdom)
A. C. Mitchell, Univ. of Salford (United Kingdom)
J. G. Murray, Univ. of Salford (United Kingdom)

Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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