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Proceedings Paper

Long-range energy transfer on DNA substrate
Author(s): Christopher R. Guest; Shiow Meei Chen; F. E. Heffron; David P. Millar
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Paper Abstract

The technique of long-range electronic energy transfer has been used to obtain structural data on nucleic acids in solution. Fluorescent chromophores have been covalently attached via an aminoethyl linker to the 5'-terminus of a synthetic oligonucleotide. Duplexes were formed with an energy donor, fluorescein, at one end and an acceptor, tetramethyl rhodamine, at the other. The rate of energy transfer has been determined from the fluorescence decay of the donor which was measured with a picosecond photon counting system. The effects of dye:DNA interactions cause additional quenching of the donor fluorescence and must be considered in the interpretation of the energy transfer experiments. An apparent donor:acceptor distance of 40.9 angtroms was calculated for a 12 b.p. labelled duplex.

Paper Details

Date Published: 1 May 1990
PDF: 6 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17769
Show Author Affiliations
Christopher R. Guest, Research Institute of Scripps Clinic (United States)
Shiow Meei Chen, Research Institute of Scripps Clinic (United States)
F. E. Heffron, Research Institute of Scripps Clinic (United States)
David P. Millar, Research Institute of Scripps Clinic (United States)


Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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