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Proceedings Paper

Sequence-dependent dynamics of DNA
Author(s): David P. Millar; Larry C. Sowers
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Paper Abstract

The sequence-dependent high-frequency motion of a fluorescent base in DNA has been studied by picosecond time-resolved fluorescence depolarization measurements on a series of defined-sequence synthetic oligonucleotides of the type d[GCCXCCG] d[CGG(AP)GGC], where X = T, A, G or C and AP is the fluorescent adenine analogue 2- aminopurine. The fluorescence anisotropy contained a rapidly decaying component that was due to restricted internal rotation of AP and a slowly decaying component due to overall tumbling. The tumbling correlation time varied with X in a manner that reflected the association state of the oligonucleotide strands. The internal motion dynamics of AP depended on the nature of the base-pairing interactions between AP and x.

Paper Details

Date Published: 1 May 1990
PDF: 7 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17768
Show Author Affiliations
David P. Millar, Research Institute of Scripps Clinic (United States)
Larry C. Sowers, City of Hope National Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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