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Proceedings Paper

Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry
Author(s): Joseph R. Lakowicz; Wieslaw M. Wiczk; Ignacy Gryczynski; Henryk Szmacinski; Michael L. Johnson
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Paper Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities of 0.6 - 200cP were obtained using propylene glycol at different temperatures (0-80°C) and methanol at 20°C. The intensity decays of naphthalene were measured in frequency-domain. Several theoretical models, including distance distributions were used to fit the data. The results indicate that end-to-end diffusion of flexible donor - acceptor pairs can be readily detected and quantified using frequency-domain fluorometry.

Paper Details

Date Published: 1 May 1990
PDF: 16 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17748
Show Author Affiliations
Joseph R. Lakowicz, Univ. of Maryland (United States)
Wieslaw M. Wiczk, Univ. of Maryland (United States)
Ignacy Gryczynski, Univ. of Maryland (United States)
Henryk Szmacinski, Univ. of Maryland (United States)
Michael L. Johnson, Univ. of Virginia (United States)


Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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