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Proceedings Paper

Investigation of ground state interaction through fluorescence decay surfaces of excited state reactions
Author(s): Marcel Ameloot; Noel Boens
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Paper Abstract

fluorescence decay surfaces of excited state reactions can be globally analyzed directly in terms of reaction rates and species associated spectra [Beechem et al., Chem. Phys. Letters 120 (1985) 466]. The identifiability of two-state excited state reactions has been investigated for properly normalized decay curves assuming that the ratio of the ground state absorbances is known [Ameloot et al, Chem. Phys. Letters 129 [1986] 21 1]. It is demonstrated in this paper that the condition of proper normalization is not always required. In addition, it is shown that the ratio of the absorbances of the species in the ground state can be obtained from fluorescence decay surfaces. The required experimental design is indicated.

Paper Details

Date Published: 1 May 1990
PDF: 8 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17741
Show Author Affiliations
Marcel Ameloot, Limburgs Univ. Centrum (Belgium)
Noel Boens, Catholic Univ. Leuven (Belgium)


Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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