Share Email Print
cover

Proceedings Paper

Time-resolved phosphorescence of proteins: structural studies using energy transfer in the rapid diffusion limit
Author(s): Ari Gafni; Joseph V. Mersol; Duncan G. Steel
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Room temperature phosphorescence emitted by tryptophan residues in deoxygenated aqueous solutions of proteins is extremely sensitive to the environment of these residues and can be utilized for the detailed study of protein structure and dynamics. The long decay time of their triplet state makes the phosphorescent tryptophans suitable donors for resonance energy transfer in the rapid diffusion limit. As shown by Stryer et al. (Ann. Rev. Biophys. Bioeng. 11, 203 (1982)) proper data analysis can then yield the distance of closest approach between the donor-acceptor pair. This method can thus allow one to map the distances of phosphorescent tryptophans from the surface of the protein. In the present study a laser-based photon counting system was used to follow the room-temperature phosphorescence decays of alkaline phosphatase and horse liver alcohol dehydrogenase and to study the quenching of their triplet states by several molecules whose absorption spectra overlap the long-lived emission of these proteins. The results demonstrate the potential applicability of these measurements for the mapping of phosphorescent tryptophan residues and confirm the phosphorescence of alkaline phosphatase to originate in Trp-109. Limitations to the applicability of the energy-transfer approach arise from quenching mechanisms which compete with resonance transfer. Two such processes - electron transfer and exchange interactions - are discussed.

Paper Details

Date Published: 1 May 1990
PDF: 12 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17721
Show Author Affiliations
Ari Gafni, Univ. of Michigan (United States)
Joseph V. Mersol, Univ. of Michigan (United States)
Duncan G. Steel, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

© SPIE. Terms of Use
Back to Top