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Proceedings Paper

Two-photon electronic spectra of nucleotides
Author(s): Scott Williams; Patrik R. Callis
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Paper Abstract

Polarized two-photon fluorescence excitation spectra were obtained for the nucleotides TMP, CMP, GMP, and AMP in neutral aqueous room temperature solution (0.05M). The wavelength range was 400- 600 nm, corresponding to states reached with 200-300 nm single UV photons. INDO/S-CI predictions were carried out for the corresponding methyl bases, and were somewhat useful for the 260 nm bands with certain parameterizations. Theory failed to find the strong two-photon absorption seen below 450 nm, which may be of Rydberg origin.

Paper Details

Date Published: 1 May 1990
PDF: 12 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17699
Show Author Affiliations
Scott Williams, Montana State Univ. (United States)
Patrik R. Callis, Montana State Univ. (United States)


Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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