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Proceedings Paper

Video imaging of cardiac transmembrane activity
Author(s): William T. Baxter; Jorge Davidenko; Candido Cabo; Jose Jalife
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Paper Abstract

High resolution movies of transmembrane electrical activity in thin (0.5 mm) slices of sheep epicardial muscle were recorded by optical imaging with voltage-sensitive dyes and a CCD video camera. Activity was monitored at approximately 65,000 picture elements per 2 cm2 tissue for several seconds at a 16 msec sampling rate. Simple image processing operations permitted visualization and analysis of the optical signal, while isochrome maps depicted complex patterns of propagation. Maps of action potential duration and regional intermittent conduction block showed that even these small preparations may exhibit considerable spatial heterogeneity. Self-sustaining reentrant activity in the form of spiral waves was consistently initiated and observed either drifting across the tissue or anchored to small heterogeneities. The current limitations of video optical mappings are a low signal-to- noise ratio and low temporal resolution. The advantages include high spatial resolution and direct correlation of electrical activity with anatomy. Video optical mapping permits the analysis of the electrophysiological properties of any region of the preparation during both regular stimulation and reentrant activation, providing a useful tool for studying cardiac arrhythmias.

Paper Details

Date Published: 26 May 1994
PDF: 10 pages
Proc. SPIE 2132, Clinical Applications of Modern Imaging Technology II, (26 May 1994); doi: 10.1117/12.176577
Show Author Affiliations
William T. Baxter, SUNY Health Science Ctr. (United States)
Jorge Davidenko, SUNY Health Science Ctr. (United States)
Candido Cabo, SUNY Health Science Ctr. (United States)
Jose Jalife, SUNY Health Science Ctr. (United States)


Published in SPIE Proceedings Vol. 2132:
Clinical Applications of Modern Imaging Technology II
Leonard J. Cerullo M.D.; Kenneth S. Heiferman M.D.; Hong Liu; Halina Podbielska M.D.; Abund Ottokar Wist; Lucia J. Zamorano, Editor(s)

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