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Proceedings Paper

Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury
Author(s): Tayyaba Hasan; M. E. Sherwood; T. Anderson; Mike Bamberg; Thomas J. Flotte; Vince R. Zurawski
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Paper Abstract

An anti-ovarian carcinoma antibody OC125 was conjugated to a derivative of the photosensitizer (PS) chiorin e6 yj polyglutamic acid. Target cells from a human ovarian cancer cell line were treated with this conjugate and laser irradiation at 656 rim (absorption maximum of PS) and fixed 24 h later for electron microscopy. Electron niicrographs showed a high degree of vacoulization, generalized cell necrosis, and extrusion of organelles. No specific damage to the plasma membrane was noted. Untreated control cells, or cells treated with conjugate or light alone exhibited no injury. These data suggest that even though the antibody recognizes a cell surface antigen, the conjugate is internalized under the conditions of the experiment.

Paper Details

Date Published: 1 July 1990
PDF: 8 pages
Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); doi: 10.1117/12.17657
Show Author Affiliations
Tayyaba Hasan, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
M. E. Sherwood, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
T. Anderson, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Mike Bamberg, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Thomas J. Flotte, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Vince R. Zurawski, Centocor and Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 1203:
Photodynamic Therapy: Mechanisms II
Thomas J. Dougherty, Editor(s)

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