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Proceedings Paper

Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells
Author(s): Christopher R. Shea; Diana Whitaker; George F. Murphy; Norah Chen; Joanne Wimberly; M. Scholz; M. E. Sherwood; Thomas J. Flotte; Tayyaba Hasan
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Paper Abstract

In order to elucidate the mechanisms of photosensitized injury to mitochondria, two photosensitizers have been compared. Both doxycycline (DOTC) and rhodamine-l23 (R123) localize preferentially within the mitochondria of MGH-IJ1 bladder carcinoma cells j1 vitro, and both sensitize phototoxic injury that is selective for mitochondria. Mitochondria of cells pretreated with DOTC and irradiated with UVA (1 J/cm2, 320-400 rim) undergo massive swelling that begins by 10 mm after irradiation, is maximal by 1 h, and is partially repaired by 4 h; damage caused by exposure to a higher UVA dose (6 J/cm2), however, is not repaired. In contrast, cells pretreated with R123 and irradiated with an argon-ion laser (10 J/cm2, 514.5 rim) undergo a different type of mitochondrial injury, characterized by the delayed (4 h) onset of moderate mitochondrial swelling and striking mitochondrial distortion and fragmentation, which is not repaired by 48 h after irradiation. These differences indicate that the reactions underlying cellular phototoxicity can be distinguished even on an ultrastructural level. Probably both the primary photochemistry and the submitochondrial targets of these reactions differ with the two photosensitizers.

Paper Details

Date Published: 1 June 1990
PDF: 8 pages
Proc. SPIE 1202, Laser-Tissue Interaction, (1 June 1990); doi: 10.1117/12.17613
Show Author Affiliations
Christopher R. Shea, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Diana Whitaker, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
George F. Murphy, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Norah Chen, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Joanne Wimberly, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
M. Scholz, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
M. E. Sherwood, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Thomas J. Flotte, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)
Tayyaba Hasan, Wellman Labs. of Photomedicine, Harvard Medical School and Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 1202:
Laser-Tissue Interaction
Steven L. Jacques, Editor(s)

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