Share Email Print
cover

Proceedings Paper

Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy
Author(s): Christina L. Burch; Joseph R. Lakowicz; Eva Marie Sevick-Muraca
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Numerical solutions to the diffusion equation describing light propagation in tissues are presented. The results show that time- dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a fluorophore uniformly distrusted in tissues. With proper referencing, frequency-domain measurements of phase-shift, (Theta) , may allow quantitation of fluorescent lifetimes, (tau) , independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetimes, (tau) .

Paper Details

Date Published: 19 May 1994
PDF: 14 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176003
Show Author Affiliations
Christina L. Burch, Vanderbilt Univ. (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Eva Marie Sevick-Muraca, Vanderbilt Univ. (United States)


Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

© SPIE. Terms of Use
Back to Top