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Proceedings Paper

Interferometric versus confocal techniques for imaging microstructures in turbid biological media
Author(s): Joseph M. Schmitt; Alexander R. Knuettel; Michael J. Yadlowsky
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Paper Abstract

In this paper we compare the performance of confocal and optical- coherence (OC) microscopes designed for imaging structures in a dense biological tissue, like skin, to depths greater than several hundred micrometers. Simple theoretical models, supplemented by Monte-Carlo simulations, are developed for evaluating the optical-sectioning capabilities of the two types of microscopes. The OC microscope is shown to exhibit superior rejection of undesired scattered light when the available angular field of view is restricted. Results of experimental studies with tissue phantoms show a progressive degradation with optical depth in the contrast of objects viewed by a confocal microscope compared to that achieved with the heterodyne technique. We conclude by making a few observations and generalizations regarding the suitability of OC and confocal techniques for potential in-vivo applications.

Paper Details

Date Published: 19 May 1994
PDF: 12 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.175999
Show Author Affiliations
Joseph M. Schmitt, National Institutes of Health (United States)
Alexander R. Knuettel, National Institutes of Health (United States)
Michael J. Yadlowsky, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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