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Proceedings Paper

In-vivo fluorescence detection and imaging of porphyrin-producing bacteria in the human skin and in the oral cavity for diagnosis of acne vulgaris, caries, and squamous cell carcinoma
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Paper Abstract

Certain bacteria are able to synthesize metal-free fluorescent porphyrins and can therefore be detected by sensitive autofluorescence measurements in the red spectral region. The porphyrin-producing bacterium Propionibacterium acnes, which is involved in the pathogenesis of acne vulgaris, was localized in human skin. Spectrally resolved fluorescence images of bacteria distribution in the face were obtained by a slow-scan CCD camera combined with a tunable liquid crystal filter. The structured autofluorescence of dental caries and dental plaque in the red is caused by oral bacteria, like Bacteroides or Actinomyces odontolyticus. `Caries images' were created by time-gated imaging in the ns-region after ultrashort laser excitation. Time-gated measurements allow the suppression of backscattered light and non-porphyrin autofluorescence. Biopsies of oral squamous cell carcinoma exhibited red autofluorescence in necrotic regions and high concentrations of the porphyrin-producing bacterium Pseudomonas aerigunosa. These studies suggest that the temporal and spectral characteristics of bacterial autofluorescence can be used in the diagnosis and treatment of a variety of diseases.

Paper Details

Date Published: 19 May 1994
PDF: 10 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.175988
Show Author Affiliations
Karsten Koenig, Beckman Laser Institute and Medical Clinic (United States)
Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)
Herbert Schneckenburger, Univ. Ulm (Germany)
Joerg Hemmer, Univ. Ulm (Germany)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Rudolf W. Steiner, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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