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Proceedings Paper

Multidisciplinary approach for detecting subcellular objects in low level light fluorescence microscopy
Author(s): Amir Waks; Hershel Ginsburg
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Paper Abstract

In the emerging field of biotechnology, new technologies that enable early detection of genetic aberration or cancer are becoming a reality. These methods label specific parts of the DNA of the chromosomes with fluorescence fluors. The development of the techniques, as well as the correlation of the genetic aberrations to cancer, require a scientific imaging system coupled with an advanced image analysis method. In this paper we outline the problems associated with both the imaging and image analysis. We present a coherent approach to the design of the system. The system parameters are considered simultaneously so that the acquisition process is fast, yet the image quality is good so that its analysis is reliable. A fast new technique for scanning/focusing using extrapolation and interpolation is described. Moreover, a novel approach for a robust analysis through a combination of morphological operators and statistical optimization is outlined. Experiments and results that evaluate the system performance are presented.

Paper Details

Date Published: 5 May 1994
PDF: 9 pages
Proc. SPIE 2173, Image Acquisition and Scientific Imaging Systems, (5 May 1994); doi: 10.1117/12.175171
Show Author Affiliations
Amir Waks, Amoco Technology Co. (United States)
Hershel Ginsburg, Amoco Technology Co. (United States)


Published in SPIE Proceedings Vol. 2173:
Image Acquisition and Scientific Imaging Systems
Helen C. Titus; Amir Waks, Editor(s)

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