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Proceedings Paper

Biotinylated polyalkylthiophene thin films and monolayers that specifically incorporate phycobiliproteins: toward smart materials
Author(s): Madhu Sudhan Rao Ayyagari; Rajiv Pande; Jeong Ok Lim; Manohar Kamath; Nagendra Beladakere; Harry Hong Gao; Kenneth A. Marx; Sukant K. Tripathy; Jayant Kumar; Lynne A. Samuelson; Joseph A. Akkara; David L. Kaplan
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Paper Abstract

We are investigating thin film and monolayer systems that involve conjugated conducting polymers and specific biological macromolecules. One class of conducting polymers, polyalkylthiophenes, are derivatized with biotin. These biotinylated polymers form the basis for a generic cassette system of attachment for any biological molecule through biotinylation or interaction with streptavidin. The high affinity of the biotin-streptavidin system, used in sequential steps, forms the basis of the cassette method. We have formed both monolayers and thin films (a few nanometers) of the cassette assembly in which phycobiliproteins are incorporated. We are investigating the optical signal transduction properties of specific phycobiliproteins (phycoerythrin, phycocyanin and allophycocyanain) using the cassette system on the inner surface of glass capillaries and on optical fiber surfaces. Phycobiliprotein photocurrent signals in conducting polymer matrices on microelectrodes are also being investigated. Our aim is to integrate the signal transduction mechanisms of the phycobiliproteins within monolayers or thin films of the conducting polymers to create biosensors and related smart materials for applications in biomedicine and biotechnology.

Paper Details

Date Published: 1 May 1994
PDF: 10 pages
Proc. SPIE 2189, Smart Structures and Materials 1994: Smart Materials, (1 May 1994); doi: 10.1117/12.174048
Show Author Affiliations
Madhu Sudhan Rao Ayyagari, Univ. of Massachusetts/Lowell (United States)
Rajiv Pande, Univ. of Massachusetts/Lowell (United States)
Jeong Ok Lim, Univ. of Massachusetts/Lowell (United States)
Manohar Kamath, Univ. of Massachusetts/Lowell (United States)
Nagendra Beladakere, Univ. of Massachusetts/Lowell (United States)
Harry Hong Gao, Univ. of Massachusetts/Lowell (United States)
Kenneth A. Marx, Univ. of Massachusetts/Lowell (United States)
Sukant K. Tripathy, Univ. of Massachusetts/Lowell (United States)
Jayant Kumar, Univ. of Massachusetts/Lowell (United States)
Lynne A. Samuelson, U.S. Army Natick Research, Development & Engineering (United States)
Joseph A. Akkara, U.S. Army Natick Research, Development & Engineering Ctr. (United States)
David L. Kaplan, U.S. Army Natick Research, Development & Engineering Ctr. (United States)

Published in SPIE Proceedings Vol. 2189:
Smart Structures and Materials 1994: Smart Materials
Vijay K. Varadan, Editor(s)

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