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Proceedings Paper

Two photon excitation in time-resolved fluorescence microscopy
Author(s): Pekka E. Hanninen; Stefan W. Hell; Ari Kuusisto; Lotta Lehtela; Juha Salo; Erkki J. Soini
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Paper Abstract

Resonant two photon excitation of UV absorbing dyes with red light is a new concept in 3D microscopy. This technique provides functionality which is virtually equivalent to a confocal microscope. Two photon excitation allows excitation of UV dyes without special UV corrected optics. Resonant two photon excitation in microscopy, as known until now, utilizes expensive, high powered pulsed femto- or picosecond lasers. In this paper a cost effective alternative to resonant two photon is presented.

Paper Details

Date Published: 4 April 1994
PDF: 6 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172107
Show Author Affiliations
Pekka E. Hanninen, Univ. of Turku (Finland)
Stefan W. Hell, Univ. of Turku (Finland)
Ari Kuusisto, Univ. of Turku (Finland)
Lotta Lehtela, Univ. of Turku (Finland)
Juha Salo, Univ. of Turku (Finland)
Erkki J. Soini, Univ. of Turku (Finland)

Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

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