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Proceedings Paper

Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy
Author(s): Brent Bailey; Vijay Krishnamurthi; Daniel L. Farkas; D. Lansing Taylor; Frederick Lanni
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Paper Abstract

Axial resolution in fluorescence microscopy can be improved significantly by using standing wave illumination to selectively excite planes within the depth of field of the microscope. When the specimen is thinner than 0.18 micrometers , an estimate of its 3D structure may be determined from three images within the same focal plane without re-focusing. Thicker objects require a combination of multi-focal-plane data and/or a priori knowledge.

Paper Details

Date Published: 4 April 1994
PDF: 6 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172101
Show Author Affiliations
Brent Bailey, Carnegie Mellon Univ. (United States)
Vijay Krishnamurthi, Carnegie Mellon Univ. (United States)
Daniel L. Farkas, Carnegie Mellon Univ. (United States)
D. Lansing Taylor, Carnegie Mellon Univ. (United States)
Frederick Lanni, Carnegie Mellon Univ. (United States)


Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

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