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Proceedings Paper

Motion estimation and visualization for four-dimensional optical microscopy
Author(s): Wallace Frank Marshall; David A. Agard; John W. Sedat
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Paper Abstract

Four-dimensional (4D) microscopy, the acquisition of time-resolved 3D images of living biological samples, provides not only structural data but also contains information on the mechanics and forces inside a living cell. In order to extract, quantify, and visualize such information for chromosomes, we are exploring simple computational methods that will allow quantitative estimation of chromosome motion within 4D images of living nuclei. A correspondence-based approach is required due to limited temporal resolution. The chromosomes we are tracking are relatively textureless, but additional structural constraints can be imposed to discriminate against false matches.

Paper Details

Date Published: 4 April 1994
PDF: 10 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172088
Show Author Affiliations
Wallace Frank Marshall, Univ. of California/San Francisco (United States)
David A. Agard, Univ. of California/San Francisco (United States)
John W. Sedat, Univ. of California/San Francisco (United States)


Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

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