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Proceedings Paper

Time-correlated single-photon counting using a confocal scanning laser microscope
Author(s): Hjalmar Brismar
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Paper Abstract

A system for 3D lifetime measurements of fluorophores has been developed and applied to multiple stained specimens. The system is built around a picosecond detector system for time correlated single photon counting and a confocal scanning laser microscope. It is capable of measuring lifetimes in the interval 1 - 20 ns, with a resolution of approximately 10 ps. By studying the lifetime of the fluorescence in a specimen it is possible to identify different fluorophores even if their emission spectra overlap. In addition, information regarding the chemical environment may be obtained due to effects from the environment on the fluorescence lifetime of the fluorophore.

Paper Details

Date Published: 4 April 1994
PDF: 11 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172081
Show Author Affiliations
Hjalmar Brismar, Royal Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

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