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Proceedings Paper

Phase-contrast microscopy in the in-vitro study of neoplastic cell dynamic morphology and behavior as related to malignancy
Author(s): Pavel Vesely
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Paper Abstract

Phase contrast microscopy plays a major role in establishing knowledge on in vitro cell behavior in cultured cells. A major focus of these studies has been on the identification of differences between normal and neoplastic cells. Spontaneously metastasizing rat sarcoma was studied to determine the relationship between the rate of appearance of spontaneous metastases and the patterns of in vitro cell behavior. It was found that a slightly acid extracellular milieu (pH 6.5), simulating the natural situation within the tumor, brought about stimulation of directional migration in highly malignant phenotypes. That type of behavior may account for malignant spread of a neoplastic cell from the primary site of the tumor. A set of the in vitro observable properties of the malignant cell phenotype is detailed here and called activated morphotype. A working theory about the origin of the activated morphotype of the neoplastic cell (AMNC) is presented.

Paper Details

Date Published: 3 May 1994
PDF: 6 pages
Proc. SPIE 1846, Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications, (3 May 1994); doi: 10.1117/12.171869
Show Author Affiliations
Pavel Vesely, Institute of Molecular Genetics (Czech Republic)

Published in SPIE Proceedings Vol. 1846:
Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications
Maksymilian Pluta; Mariusz Szyjer, Editor(s)

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