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Proceedings Paper

Antibody-based bacterial toxin detection
Author(s): Darrell E. Menking; Jonathon M. Heitz; Nabil A. Anis; Roy G. Thompson
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Paper Abstract

Fiber optic evanescent fluorosensors are under investigation in our laboratory for the study of drug-receptor interactions for detection of threat agents and antibody-antigen interactions for detection of biological toxins. In a one step assay, antibodies against Cholera toxin or Staphylococcus Enterotoxin B were noncovalently immobilized on quartz fibers and probed with fluorescein-isothiocyanate (FITC)-labeled toxins. In the two-step assay, Cholera toxin or Botulinum toxoid A was immobilized onto the fiber, followed by incubation in an antiserum or partially purified antitoxin IgG. These were then probed with FITC-anti-IgG antibodies. Unlabeled toxins competed with labeled toxins or antitoxin IgG in a dose-dependent manner and the detection of the toxins was in the nanomolar range.

Paper Details

Date Published: 10 March 1994
PDF: 6 pages
Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); doi: 10.1117/12.170668
Show Author Affiliations
Darrell E. Menking, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
Jonathon M. Heitz, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
Nabil A. Anis, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
Roy G. Thompson, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)


Published in SPIE Proceedings Vol. 2068:
Chemical, Biochemical, and Environmental Fiber Sensors V
Robert A. Lieberman, Editor(s)

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