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Proceedings Paper

Determination of the kinetic response and absolute sensitivity of a fiber-optic immunoassay
Author(s): Sandra F. Feldman; E. E. Uzgiris
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Paper Abstract

We have used an evanescently excited fiber optic immunoassay to study the kinetic response of an immunosensor and determine its absolute sensitivity. We have estimated that when binding sites are saturated approximately 1011 antigen are bound to a fiber probe of 0.57 cm2 active area using optical techniques and without resorting to a radioassay. The number of labeled antigen for a threshold signal is approximately 109. We can easily detect fluorescently labeled antigen at subnanomolar concentrations and have measured the probe response to antigens over more than two orders of magnitude. The overall response time for this system and labeled antigen (Mw approximately equals 100,000) is on the order of 10 minutes for a 40 pM solution. At higher concentrations, a response may be obtained in a few minutes.

Paper Details

Date Published: 10 March 1994
PDF: 6 pages
Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); doi: 10.1117/12.170659
Show Author Affiliations
Sandra F. Feldman, GE Corporate Research and Development Ctr. (United States)
E. E. Uzgiris, GE Corporate Research and Development Ctr. (United States)

Published in SPIE Proceedings Vol. 2068:
Chemical, Biochemical, and Environmental Fiber Sensors V
Robert A. Lieberman, Editor(s)

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