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Proceedings Paper

Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science
Author(s): Joachim Wietzorrek; E. Glossner; R. Kuehn; V. Kachel
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Paper Abstract

The procedure of imaging in flow, evoked by the need for simplified and reliable particle identification in aquatic science, means taking cell images directly from the transducer of a flow cytometer. From that point of view imaging in flow is the link of the traditional microscopical method and the modern flow cytometric technique for analyzing particles. Principal problems rising from that approach are discussed in this paper. This encloses positioning of the objects to be imaged, design criteria for illumination and imaging optics, the procedure for taking asynchronous video images and requirements concerning the light source.

Paper Details

Date Published: 15 February 1994
PDF: 13 pages
Proc. SPIE 2085, Biochemical and Medical Sensors, (15 February 1994); doi: 10.1117/12.168746
Show Author Affiliations
Joachim Wietzorrek, Max-Planck-Institute (Germany)
E. Glossner, Max-Planck-Institute (Germany)
R. Kuehn, Max-Planck-Institute (Germany)
V. Kachel, Max-Planck-Institute (Germany)


Published in SPIE Proceedings Vol. 2085:
Biochemical and Medical Sensors
Otto S. Wolfbeis, Editor(s)

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