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Proceedings Paper

Uptake of zinc(II)-phthalocyanine by HepG2 cells expressing the low-density lipoprotein receptor: studies with the liposomal formulation CGP55847
Author(s): William G. Love; Ellen C. Havenaar; Philip J. Lowe; Peter W. Taylor
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Paper Abstract

Hydrophobic photosensitizers readily intercalate into plasma lipoproteins. Some tumors acquire cholesterol from the circulation as a result of increased low density lipoprotein (LDL) receptor activity. Thus, circulating LDL may function as a vehicle for the delivery of bound Zn-Pc to cells within a tumor. Zn-Pc:LDL complexes, resulting from the interaction of LDL with the liposomal Zn-Pc formulation CGP55847, bind to the LDL receptor expressed on HepG2 cells but with reduced affinity in comparison to LDL. Confocal fluorescence microscopy facilitated the subcellular localization of Zn-Pc in microcolonies of HepG2 cells; the photosensitizer was distributed throughout the cellular membrane systems but was absent from the cell nucleus. Uptake of Zn-Pc in the presence of LDL was twofold greater than in the absence of the lipoprotein. These data suggest that the LDL uptake pathway may contribute to the localization of Zn-Pc in hyperproliferative tissue.

Paper Details

Date Published: 1 March 1994
PDF: 8 pages
Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); doi: 10.1117/12.168678
Show Author Affiliations
William G. Love, CIBA-GEIGY Pharmaceuticals (United Kingdom)
Ellen C. Havenaar, CIBA-GEIGY Pharmaceuticals (United Kingdom)
Philip J. Lowe, CIBA-GEIGY Pharmaceuticals (United Kingdom)
Peter W. Taylor, CIBA-GEIGY Pharmaceuticals (United Kingdom)

Published in SPIE Proceedings Vol. 2078:
Photodynamic Therapy of Cancer
Giulio Jori; Johan Moan; Willem M. Star, Editor(s)

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