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Proceedings Paper

Optical methods of investigation of the protein Langmuir: Blodgett films
Author(s): Oleg I. Lobanov; T. B. Dubrovsky; Alexander P. Savitsky; D. E. Akindinov; Ilya V. Alexeev; V. V. Savransky
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Paper Abstract

The structure of multilayer Langmuir-Blodgett (LB) films made of protein (Bovine serum albumine)-co-proporphyrin(III) conjugate was investigated by means of fluorescence microscope coupled with the time-correlated single photon counting registration system. The map of coproporphyrin fluorescence from the surface of protein LB-films was measured with picosecond time resolution and spatial resolution 15 micrometers . The sample area 240 X 240 micrometers was irradiated step by step by a focused beam of Nd:YAG laser with an accumulation time of 3 sec per point. There were two different kinds of samples: those with regular and those with irregular distribution of fluorescent labels over the sample.

Paper Details

Date Published: 1 February 1994
PDF: 5 pages
Proc. SPIE 2082, Quantification and Localization Using Diffuse Photons in a Highly Scattering Medium, (1 February 1994); doi: 10.1117/12.167471
Show Author Affiliations
Oleg I. Lobanov, Institute of Biochemistry (Russia)
T. B. Dubrovsky, Institute of Biochemistry (Russia)
Alexander P. Savitsky, Institute of Biochemistry (Russia)
D. E. Akindinov, Moscow State Univ. (Russia)
Ilya V. Alexeev, Institute of Crystallography (Russia)
V. V. Savransky, General Physics Institute (Russia)


Published in SPIE Proceedings Vol. 2082:
Quantification and Localization Using Diffuse Photons in a Highly Scattering Medium
Britton Chance; David T. Delpy; Marco Ferrari; Martin J. C. van Gemert; Gerhard J. Mueller; Valery V. Tuchin, Editor(s)

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