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Proceedings Paper

Fourier transform infrared microspectroscopy shows significant differences between spectra of undifferentiated and polynucleated FLG 29.1 dried cells
Author(s): Salvatore Romano; Susanna Benvenuti; Antonio Conti; Enzo Benedetti; Emilia Bramanti; Ilaria Rossi; Edoardo Benedetti
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Paper Abstract

In a recent study made on cultures of human leukaemic cells (FLG 29.1 cell line) we were able to detect, by IR microspectroscopy, some significant IR spectroscopic variations following differentiation of cells towards osteoclastic-like behavior. The present study was undertaken on the same cell line in order to monitor biochemical structure variations following fusion induced by polyetilenglycole (PEG), using FTIR microspectroscopy. The finger-print region of all the spectra was retained and normalized according to a new regression procedure. Eleven bands were selected and total band power and mean power per unit frequency were compared with the corresponding reference session bands by a Dunnett's T test. Significant differences were found in both the tested variables only between treated and untreated cells, in 6 bands.

Paper Details

Date Published: 1 February 1994
PDF: 6 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167438
Show Author Affiliations
Salvatore Romano, Univ. di Firenze (Italy)
Susanna Benvenuti, Univ. di Firenze (Italy)
Antonio Conti, Univ. di Firenze (Italy)
Enzo Benedetti, Univ. di Pisa (Italy)
Emilia Bramanti, Univ. di Pisa (Italy)
Ilaria Rossi, Univ. di Pisa (Italy)
Edoardo Benedetti, Univ. di Pisa (Italy)


Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska; Herbert Schneckenburger; Tony Wilson, Editor(s)

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