Share Email Print

Proceedings Paper

Methods and application of time-resolved fluorescence microscopy
Author(s): Harold K. Seidlitz; Jurina M. Wessels
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The combination of fluorescence microscopy and time-resolved techniques opens up new possibilities in studying pharmacokinetics and localization of various drugs on a cellular scale. The fluorescence decay time of many molecules is modulated by the physical and chemical properties of the microenvironment, such as viscosity, polarity, pH-value, etc. The alternation of the decay time can also be used to pursue chemical transformations of a drug like degradation, monomerization, or protonation. This paper presents applications of this method from the field of photosensitizing dyes used in photodynamic therapy.

Paper Details

Date Published: 1 February 1994
PDF: 9 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167426
Show Author Affiliations
Harold K. Seidlitz, GSF-Forschungszentrum fuer Umwelt und Gesundheit (Germany)
Jurina M. Wessels, GSF-Forschungszentrum fuer Umwelt und Gesundheit (Germany)

Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska; Herbert Schneckenburger; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top