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Proceedings Paper

Video microscopy of vascular damage during photodynamic therapy
Author(s): Sol Kimel; Varda Gottfried
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Paper Abstract

Photodynamic therapy (PDT) was performed in the chorioallantoic membrane (CAM) of the chick embryo. This is a convenient model to study vascular effects. PDT-induced damage was monitored continuously during irradiation and recorded on a VCR driven by a PC. Time-lapse video documentation provided a detailed view of the entire process: tumor growth, angiogenesis, vascularization, PDT, and tumor regression. Our particular interest was in resolving the controversy regarding the primary mechanism in PDT, as to whether the predominant damage is to the parenchymal tumor cells or to the vascular endothelium. Image analysis techniques enabled us to follow quantitatively changes occurring during the process. These changes included alternations in blood vessels (color and morphology) and in tumors (tumor area).

Paper Details

Date Published: 1 February 1994
PDF: 7 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167425
Show Author Affiliations
Sol Kimel, Technion--Israel Institute of Technology (Israel)
Varda Gottfried, Technion--Israel Institute of Technology (Israel)


Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska; Herbert Schneckenburger; Tony Wilson, Editor(s)

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