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Proceedings Paper

Dependency of subcellular reactions during PDT on the metabolic state of cell cultures probed by different microscopic techniques
Author(s): Angelika C. Rueck; Herbert Schneckenburger; Wolfgang S. L. Strauss; Michael H. Gschwend; Gerd C. Beck; Karin Kunzi-Rapp; Rudolf W. Steiner
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Paper Abstract

Various microscopic techniques were used to study the dependency of photodynamically induced subcellular reactions on the metabolic state of cell cultures. TPPS4 and AlS2-3Pc were incubated in RR 1022 epithelial cells with varying cell density. To attain almost isolated cells (low cell density) or confluent growing cells (high cell density) 25 cells/mm2 or 500 cells/mm2 were seeded, respectively. Low cell density irradiation with blue light led to a change in the initial cytoplasmatic fluorescence pattern. For both sensitizers, TPPS4 as well as AlS2-3, a fluorescence relocalization and fluorescence intensity increase could be detected, moreover in the case of TPPS4 a fluorescence formation in the nucleus and nucleoli were detected. In contrast, for confluent growing cells no redistribution was observed.

Paper Details

Date Published: 1 February 1994
PDF: 9 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167424
Show Author Affiliations
Angelika C. Rueck, Univ. Ulm (Germany)
Herbert Schneckenburger, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Gerd C. Beck, Univ. Ulm (Germany)
Karin Kunzi-Rapp, Univ. Ulm (Germany)
Rudolf W. Steiner, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine

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