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Proceedings Paper

Intracellular phthalocyanine localization: confocal laser scanning microscopy studies
Author(s): Elena B. Chernyaeva; Jan Greve; Bart G. de Grooth; A. G.J.M. Van Leeuwen
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Paper Abstract

Phthalocyanines (Pc) are promising second-generation photosensitizers for the photodynamic therapy (PDT) of cancer. We report on the tetrasulfonated aluminum phthalocyanine (AlPcS4) localization in cultured Chinese hamster lung cells studied by means of confocal laser scanning microscopy (CLSM). In these cells AlPcS4 was found in granules surrounding Golgi apparatus and in the peripheral cytoplasmic region. Peripheral Pc-containing granules partially coincided with the acidic cellular compartments. The effect of irradiation with light on Pc intracellular distribution was also studied. In the Pc-free medium disruption of some Pc- containing granules was observed followed by appearance of Pc fluorescence in the cell plasma membrane, the nuclear envelope, and the near-nuclear region. When cells were irradiated in the presence of Pc in external medium a drastic increase of membrane permeability to Pc was observed, followed by Pc binding the cell plasma membrane, nuclear envelope, and some structures in the cytoplasm. Diffusive Pc fluorescence in the nucleus was also observed. The implication of observed Pc redistribution caused by irradiation with light for the PDT protocol is discussed.

Paper Details

Date Published: 1 February 1994
PDF: 9 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167421
Show Author Affiliations
Elena B. Chernyaeva, Moscow State Univ. (Russia)
Jan Greve, Univ. Twente (Netherlands)
Bart G. de Grooth, Univ. Twente (Netherlands)
A. G.J.M. Van Leeuwen, Univ. Twente (Netherlands)

Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska; Herbert Schneckenburger; Tony Wilson, Editor(s)

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