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Proceedings Paper

Measuring oxygen pressures using triplet quenching of Pd-porphine
Author(s): Michiel Sinaasappel; C. Ince; E. A. Sanderse; Hajo A. Bruining
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Paper Abstract

A non-invasive optical method for measuring free oxygen in vivo is described. The method, introduced by Wilson and co-workers, is based on the quenching of the triplet state of Pd- porphine by oxygen and is described by the Stern-Volmer relation. The quenching of the triplet state is determined by measurement of the phosphorescence decay following excitation by a pulse of light. Measuring decay times has the advantage that they are independent of the changing optical properties of tissue and concentration of the dye. We describe a setup to measure the phosphorescence decay and present the values of Kq and (tau) o as a function of pH and temperature. Furthermore, some experiments on a rat liver and gut are presented.

Paper Details

Date Published: 1 February 1994
PDF: 7 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167420
Show Author Affiliations
Michiel Sinaasappel, Erasmus Univ. of Rotterdam (Netherlands)
C. Ince, Erasmus Univ. of Rotterdam (Netherlands)
E. A. Sanderse, Erasmus Univ. of Rotterdam (Netherlands)
Hajo A. Bruining, Erasmus Univ. of Rotterdam (Netherlands)


Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska; Herbert Schneckenburger; Tony Wilson, Editor(s)

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