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Proceedings Paper

Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements
Author(s): Wolfgang Hoehne; Werner Schramm
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Paper Abstract

In vivo measurement of NADH autofluorescence is strongly disturbed by changes in the Hb absorption of excitation and fluorescence light. An approach to this problem is based on the diffuse reflectance measurement of 805 nm (isobestic point of Hb/HbO2). According a Kubelka-Munk calculation an increase in Hb/HbO2 concentration leads to a decrease of diffuse reflectance. This gives correction terms for the measured autofluorescence data. An equipment to perform such measurements will be presented.

Paper Details

Date Published: 15 January 1994
PDF: 4 pages
Proc. SPIE 2081, Optical Biopsy, (15 January 1994); doi: 10.1117/12.166826
Show Author Affiliations
Wolfgang Hoehne, Institute for Molecular Pharmacology (Germany)
Werner Schramm, Institute for Molecular Pharmacology (Germany)


Published in SPIE Proceedings Vol. 2081:
Optical Biopsy
Rinaldo Cubeddu; Sune Svanberg; Hubert van den Bergh, Editor(s)

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