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Proceedings Paper

Estimation of globular protein secondary structure by solvent elimination particle beam LC/FTIR spectrometry: correlation with other IR measurements
Author(s): Vincent E. Turula; James A. de Haseth
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Paper Abstract

To determine how well protein secondary-structure estimates obtained from particle beam spectra correlate with estimates obtained from other IR sampling methods, a series of globular protein spectra was acquired. In addition to solid film deposit produced by the particle beam, spectra were obtained by the Attenuated Total Reflectance (ATR) Circle cellTM, on ZnSe as evaporated films, and with Diffuse Reflectance in KCl. The proteins used in this study were classified into types based on relative amounts of secondary structures as determined by FT-IR spectrometry. Estimates of secondary structure content from the solid- state deposits prepared with particle beam, evaporated films, and ATR spectra were very similar. The harsh environment required for diffuse reflectance measurements may explain why estimates by this method did not compare well with the other techniques employed.

Paper Details

Date Published: 31 January 1994
PDF: 2 pages
Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166698
Show Author Affiliations
Vincent E. Turula, Univ. of Georgia (United States)
James A. de Haseth, Univ. of Georgia (United States)


Published in SPIE Proceedings Vol. 2089:
9th International Conference on Fourier Transform Spectroscopy
John E. Bertie; Hal Wieser, Editor(s)

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