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Proceedings Paper

Electroporation visualized under a multishot pulsed laser fluorescence microscope system
Author(s): Hiroyasu Itoh; Irene I. K. Yu; Masahiro Hibino; Tsuyoshi Hayakawa; Kazuhiko Kinosita
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Paper Abstract

We describe a new fluorescence microscope system, which is the third generation of our pulsed-laser microscope systems developed for the purpose of capturing rapid cellular phenomena. Time resolution of this latest version is supported by the combination of a Q- switched Nd:YAG laser producing a burst of 4 pulses and a large format framing camera. We obtain series images at intervals on the order of 10 microsecond(s) with exposure times of 30 ns. With this multi-shot pulsed laser fluorescence microscope system, we examined the behavior of the transmembrane potential in a sea urchin egg under an intense electric field. Irreversible process of cell electroporation was revealed in serial images taken under a single electric pulse of microsecond duration.

Paper Details

Date Published: 11 October 1993
PDF: 8 pages
Proc. SPIE 2002, Ultrahigh- and High-Speed Photography, Videography, and Photonics '93, (11 October 1993); doi: 10.1117/12.161357
Show Author Affiliations
Hiroyasu Itoh, Hamamatsu Photonics KK (Japan)
Irene I. K. Yu, Los Alamos National Lab. (United States)
Masahiro Hibino, Nagoya Univ. (Japan)
Tsuyoshi Hayakawa, Hamamatsu Photonics KK (Japan)
Kazuhiko Kinosita, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 2002:
Ultrahigh- and High-Speed Photography, Videography, and Photonics '93
Paul W. Roehrenbeck, Editor(s)

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