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Proceedings Paper

Optical Fourier analysis of biological cell patterns
Author(s): Barry R. Masters
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Paper Abstract

This paper presents the optical Fourier analysis of human corneal endothelial cell patterns. The quantitative analysis of human corneal endothelial cells patterns is analyzed with Fourier transform methods. The optical Fourier transform of the pattern of endothelial cell borders is analyzed to yield the average cell area and the coefficient of variation of the average cell area. The advantage of the Fourier transform analysis is the parallel processing of the optical Fourier transform and the potential development of a hybrid digital-optical device for the rapid analysis of large numbers of endothelial specular photomicrographs. The intensity distribution of the Fourier transform of binary images of endothelial cell patterns is detected with a wedge- ring detector. The radial and the angular distribution of intensity is analyzed and related to the cell size distribution in the input image.

Paper Details

Date Published: 3 September 1993
PDF: 8 pages
Proc. SPIE 1889, Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III, (3 September 1993); doi: 10.1117/12.155714
Show Author Affiliations
Barry R. Masters, Uniformed Services Univ. of the Health Sciences (United States)

Published in SPIE Proceedings Vol. 1889:
Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III
Halina Podbielska M.D., Editor(s)

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