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Proceedings Paper

Characterization of rat liver mitochondria using time-resolved spectroscopy
Author(s): Hsien-Chang Lee; NaiGuang Wang; Britton Chance
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Paper Abstract

In this study, the (mu) s' (scattering constant) and the (mu) a (absorption constant) of rat liver mitochondria were measured by time resolved spectroscopy (TRS) with a newly innovated medium matching method. Freshly isolated rat liver mitochondria were placed inside a pouch and submerged in an intralipid emulsion. Two sets of various concentrations of intralipid emulsion, one with different concentrations of intralipid and no ink and another with various concentrations of ink and a fixed intralipid concentration, were scanned for matching the (mu) s' and the (mu) a of the mitochondria. Two states of the mitochondria were scanned: the resting state (orthodox state, or state 4) and the active state (condensed state, or state 3). During the active state of the mitochondria, the active respiration of the mitochondria was initiated by the addition of either calcium chloride or ADP/succinate/Pi. Comparisons of the (mu) s' and the (mu) a were made of mitochondria at different states.

Paper Details

Date Published: 14 September 1993
PDF: 6 pages
Proc. SPIE 1888, Photon Migration and Imaging in Random Media and Tissues, (14 September 1993); doi: 10.1117/12.154642
Show Author Affiliations
Hsien-Chang Lee, Univ. of Pennsylvania School of Medicine (United States)
NaiGuang Wang, Univ. of Pennsylvania School of Medicine (United States)
Britton Chance, Univ. of Pennsylvania School of Medicine (United States)


Published in SPIE Proceedings Vol. 1888:
Photon Migration and Imaging in Random Media and Tissues
Britton Chance; Robert R. Alfano, Editor(s)

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