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Proceedings Paper

Monte Carlo modelization of laser-induced fluorescence signals in turbid media: determination of original fluorescence spectra
Author(s): Eric Tinet; Sigrid Avrillier
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Paper Abstract

In this theoretical paper we study the alterations of the laser induced fluorescence spectra as a function of the spectral variations of the absorption factor μa(λ), the scattering factor μs(λ) and of the mean cosine of the scattering angle g(λ) of the medium. The calculations of the signal received by the detector are based on a Monte Carlo simulation. All media are homogeneous, with infinite length and width, depth is assumed to be infinite. The total detected fluorescence signal S(λ) may be described by: S(λ)= F(λe,λ) . M(μae),μse),g(λe),μa(λ),μs(λ),g(λ)) where F is proportional to the original fluorescence spectrum and M a function of the optical and geometrical properties of the medium. In order to show the importance of the scattering, absorption and anisotropy spectral dependence, we have computed M for ideal experiments. Each coefficient μa, μs and g is alternately considered to be wavelength dependent while the others are considered to be constant. Consequently, each calculation provides a different distorted transfer function M(λe,λ) showing the influence of the spectral dependence of each coefficient. This signal is also different if the wavelength of the excitation light is changed.

Paper Details

Date Published: 27 August 1993
PDF: 8 pages
Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); doi: 10.1117/12.151199
Show Author Affiliations
Eric Tinet, Univ. Paris XIII (France)
Sigrid Avrillier, Univ. Paris XIII (France)

Published in SPIE Proceedings Vol. 1887:
Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods
Randall Locke Barbour; Mark J. Carvlin, Editor(s)

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