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Proceedings Paper

Three-dimensional reconstruction of a skeletal muscle cell from optical sections
Author(s): T. R. Gowrishankar; Raphael C. Lee; Chin-Tu Chen
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Paper Abstract

Fluorescence microscopy assay of cell membranes is a practical way of studying the primary mechanisms of muscle cell damage in electrical injury. The three-dimensional distribution of pores in an electric field can be measured by 3-D imaging of the distribution of a fluorescent potentiometric dye. A confocal microscope with a variable-width slit detectors, a high frequency non-mechanical scanning system and multiple-line laser illumination is highly suitable for the optical measurement of membrane potential. In this paper, the characteristics of such a confocal microscope in terms of its 3-D optical transfer function (OTF) measured using fluorescent beads is presented. 3-D reconstruction of a skeletal muscle cell stained with the potentiometric dye, di-8-ANEPPS from optical sections is also demonstrated.

Paper Details

Date Published: 29 July 1993
PDF: 6 pages
Proc. SPIE 1905, Biomedical Image Processing and Biomedical Visualization, (29 July 1993); doi: 10.1117/12.148629
Show Author Affiliations
T. R. Gowrishankar, Univ. of Chicago (United States)
Raphael C. Lee, Univ. of Chicago (United States)
Chin-Tu Chen, Univ. of Chicago (United States)

Published in SPIE Proceedings Vol. 1905:
Biomedical Image Processing and Biomedical Visualization
Raj S. Acharya; Dmitry B. Goldgof, Editor(s)

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