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Proceedings Paper

Fourier-transform mass spectrometry for high-resolution matrix-assisted laser desorption/ionization MS
Author(s): John A. Castoro; Charles L. Wilkins
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Paper Abstract

The extended ion trapping time intrinsic to the Fourier transform mass spectrometry (FTMS) experiment allows detection of the extensive fragmentation that can take place during the matrix-assisted laser desorption/ionization process. Metastable ion fragmentation undoubtedly decreases mass resolution for molecular ions in time-of-flight mass spectrometry and, because of the much longer time scale involved, does so even more dramatically in FTMS analysis. However, in this work reported here, it is shown that choice of correct experimental conditions can minimize this problem. For example, for compounds with masses lower than 2000 daltons, mass spectral resolution > 100,000 can be obtained. Spectra of a porphyrin, several proteins and an organic polymer obtained with such resolution are presented. Addition of a sugar co-matrix produced sufficiently stable bovine insulin molecular ions, m/z 5734, to obtain resolution > 25,000.

Paper Details

Date Published: 2 July 1993
PDF: 9 pages
Proc. SPIE 1857, Lasers and Optics for Surface Analysis, (2 July 1993); doi: 10.1117/12.148521
Show Author Affiliations
John A. Castoro, Univ. of California/Riverside (United States)
Charles L. Wilkins, Univ. of California/Riverside (United States)

Published in SPIE Proceedings Vol. 1857:
Lasers and Optics for Surface Analysis
Mattanjah S. de Vries, Editor(s)

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