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Proceedings Paper

Dynamic light-scattering study of monodisperse collagen in the less-dilute regime
Author(s): Tadakazu Maeda; Michiho Takasaki-Ohsita; Keiko Satoh; Mayumi Kimura
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Paper Abstract

Physicochemical properties of monodisperse type I collagen were studied using the dynamic light scattering and analytical ultra-centrifugation techniques. The measured osmotic second virial coefficient A2 is much smaller than the excluded volume limit calculated from the length L, diameter b and molecular weight M of collagen. The strength kD of the concentration dependence of the mutual diffusivity *DG of collagen is negative in the sign and large in the magnitude. The relationship kD equals 2A2M - v - k(zetz ) does not hold for collagen, while it holds for fd virus and poly((gamma) - benzyl L-glutamate), where v is the partial specific volume, and k(zetz ) the strength of the concentration dependence of the friction coefficient (zetz) . We conclude from these three lines of evidence that water is a poor solvent of collagen and that there must be very weak attractive interactions among collagen filaments which can easily be destroyed by external perturbations.

Paper Details

Date Published: 21 July 1993
PDF: 12 pages
Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); doi: 10.1117/12.148372
Show Author Affiliations
Tadakazu Maeda, Mitsubishi Kasei Institute of Life Sciences (Japan)
Michiho Takasaki-Ohsita, Mitsubishi Kasei Institute of Life Sciences (Japan)
Keiko Satoh, Kitasato Univ. (Japan)
Mayumi Kimura, Kitasato Univ. (Japan)

Published in SPIE Proceedings Vol. 1884:
Static and Dynamic Light Scattering in Medicine and Biology
Ralph J. Nossal; Robert Pecora; Alexander V. Priezzhev, Editor(s)

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