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Proceedings Paper

Dynamic light-scattering studies of internal motions of linear lambda-phage DNA in A- and B-forms
Author(s): Mariya A. Ivanova; Alexandr V. Arutyunian; Valentin A. Noskin
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Paper Abstract

Conformational changes of (lambda) -phage DNA macromolecules by dynamic light scattering (DLS) method were investigated. It was shown that the persistence length of B-form DNA could reduce to small value in some conditions, and could achieve approximately equals 12 nm. When studying the internal motions in the linear form of (lambda) -DNA, there was observed the deviation of the experimental angular dependence of the first cumulant (Gamma) from theoretical predictions for the (Gamma) in Rouse-Zimm model including hydrodynamic interactions. This deviation appears in region qRg >> 1. The first cumulant deviation to the higher frequencies is observed only for B-form DNA. Experimental behavior of the first cumulant (Gamma) for the A-form DNA coincides with the theoretical one in the Rouse-Zimm model.

Paper Details

Date Published: 21 July 1993
PDF: 9 pages
Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); doi: 10.1117/12.148369
Show Author Affiliations
Mariya A. Ivanova, Petersburg Nuclear Physics Institute (Russia)
Alexandr V. Arutyunian, Petersburg Nuclear Physics Institute (Russia)
Valentin A. Noskin, Petersburg Nuclear Physics Institute (Russia)

Published in SPIE Proceedings Vol. 1884:
Static and Dynamic Light Scattering in Medicine and Biology
Ralph J. Nossal; Robert Pecora; Alexander V. Priezzhev, Editor(s)

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