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Proceedings Paper

Dynamic light-scattering studies of mucin
Author(s): Rama Bansil; Sinisa Pajevic; Xingxiang Cao; K. Ramakrishn Bhaskar; Jeffrey Thomas LaMont; Nezham H. Afdhal; N. Niu
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Paper Abstract

Dynamic light scattering was applied to study aggregation phenomena in mucin, the glycoprotein responsible for the visco-elastic properties of mucus which is found as a lining on most epithelial cell surfaces. Intensity autocorrelation functions measured on purified mucin solutions under varying experimental conditions were analyzed by Laplace inversion methods. The results showed that at low pH (below 4) solutions of gastric mucin contain very large supra-molecular aggregates, with diffusion constants 100 times slower than those of the 2 X 106 molecular weight glycoprotein of mucin. Similar methods were used to investigate the interaction of gall bladder mucin with cholesterol-phospholipid vesicles. Repeated measurements of the intensity correlation functions after adding mucin to a suspension of vesicles showed a two-fold increase in the hydrodynamic radius of the vesicles over a period of three hours after which the vesicle size stayed constant. Control experiments with latex particles in mucin and vesicles in other proteins showed no change in size, implying that the fusion of vesicles is due to vesicle-mucin interactions.

Paper Details

Date Published: 21 July 1993
PDF: 8 pages
Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); doi: 10.1117/12.148358
Show Author Affiliations
Rama Bansil, Boston Univ. (United States)
Sinisa Pajevic, Boston Univ. (United States)
Xingxiang Cao, Boston Univ. (United States)
K. Ramakrishn Bhaskar, Boston Univ. Medical Ctr. (United States)
Jeffrey Thomas LaMont, Boston Univ. Medical Ctr. (United States)
Nezham H. Afdhal, Boston City Hospital (United States)
N. Niu, Boston City Hospital (United States)

Published in SPIE Proceedings Vol. 1884:
Static and Dynamic Light Scattering in Medicine and Biology
Ralph J. Nossal; Robert Pecora; Alexander V. Priezzhev, Editor(s)

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