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Proceedings Paper

Dynamic light-scattering studies of BSA and lysozyme using a backscatter fiber optic system
Author(s): Harbans S. Dhadwal; William W. Wilson; Rafat R. Ansari; William V. Meyer
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Paper Abstract

A comparative dynamic light scattering study of BSA and lysozyme is presented. A backscatter fiber optic system and a conventional light scattering spectrometer are used to measure the diameter of proteins in the dilute regime, that is, below concentrations of 10 mg/ml. The fiber optic system operating with a power level of 2 mW at a wavelength of 632.8 nm compares favorably with a conventional system operating with a power level of 40 mW at a wavelength of 514.5 nm. Quasi-elastic light scattering measurements taken at several concentrations demonstrate the utility of a backscatter fiber optic probe for sizing of small molecular weight proteins. The fiber probe, comprising two optical fibers, is about 3 mm in diameter and can be positioned either inside or outside the scattering cell.

Paper Details

Date Published: 21 July 1993
PDF: 9 pages
Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); doi: 10.1117/12.148349
Show Author Affiliations
Harbans S. Dhadwal, SUNY/Stonybrook (United States)
William W. Wilson, Mississippi State Univ. (United States)
Rafat R. Ansari, NASA Lewis Research Ctr. (United States)
William V. Meyer, NASA Lewis Research Ctr. (United States)


Published in SPIE Proceedings Vol. 1884:
Static and Dynamic Light Scattering in Medicine and Biology
Ralph J. Nossal; Robert Pecora; Alexander V. Priezzhev, Editor(s)

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