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Proceedings Paper

Scanned laser-induced fluorescence reproducibility
Author(s): Kenneth L. Zakariasen; Joseph R. Barron; Barry E. Paton
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Paper Abstract

Laser-induced fluorescence has potential for both caries research and diagnosis. However, research has shown that precise control is necessary for accurate enamel comparisons. Such factors as focus, number of sampling points, scan matrix size and reproducible placement of the scan matrix can all have substantial effects on results. Five noncarious third molars were mounted in acrylic and ground so that a flat Bu enamel surface was created. Each enamel area was covered with acid-resistant varnish except for a window approximately 1.0 mm2. Each specimen was mounted in the fluorometer for scanned laser fluorescence analysis using a 1.0 mm2 scan matrix. Readings were taken at 50 micrometers intervals for a total of 400 readings per matrix. Each specimen was remounted and reanalyzed in the fluorometer. Each specimen was lased (1.5 W, 1.5 mm f.s., 0.15 sec.) and reanalyzed as per above by fluorometer. Running-sums of line means were calculated for each scan and graphed. In all samples, the curves for nonlased enamel were lower and widely separated from those for lased enamel. In each specimen, the nonlased curves were either coincident or nearly so. The results indicate that ensuring constant focus, repeatable orientation of scan matrices and high sampling frequency collectively can lead to a high degree of scanned laser fluorescence reproducibility.

Paper Details

Date Published: 21 July 1993
PDF: 5 pages
Proc. SPIE 1880, Lasers in Orthopedic, Dental, and Veterinary Medicine II, (21 July 1993); doi: 10.1117/12.148328
Show Author Affiliations
Kenneth L. Zakariasen, Marquette Univ. and Dalhousie Univ. (Canada)
Joseph R. Barron, Marquette Univ. and Dalhousie Univ. (Canada)
Barry E. Paton, Marquette Univ. and Dalhousie Univ. (Canada)

Published in SPIE Proceedings Vol. 1880:
Lasers in Orthopedic, Dental, and Veterinary Medicine II
Dov Gal; Stephen J. O'Brien; C. T. Vangsness; Joel M. White; Harvey A. Wigdor, Editor(s)

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