Share Email Print
cover

Proceedings Paper

FEL microscopy for the investigation of transient local heating in single living cells
Author(s): Bruce J. Tromberg; K. A. Peterson; Tatiana B. Krasieva; Satoshi Shimizu; Chris W. Rella; Andrew Gee Jeung; Curtis F. Chapman; Dana D. Dlott; Michael D. Fayer; H. Alan Schwettman; Michael W. Berns
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

A free-electron laser (FEL) microscope has been constructed to perform spatially and spectrally resolved pump/probe experiments in single living cells. Picosecond infrared FEL pulses are absorbed by the sample and rapidly converted to heat. Excitation of localized fluorescent reporter molecules using a UV/VIS probe beam leads to thermally induced alterations in the radiative signal. Fluorescence-detected infrared (FDIR) spectra are generated from regions proximal to reporter molecules by varying the FEL pump wavelength. Sub- wavelength spatial resolution is a composite function of media thermal properties and probe selectivity.

Paper Details

Date Published: 14 July 1993
PDF: 8 pages
Proc. SPIE 1854, Free-Electron Laser Spectroscopy in Biology, Medicine, and Materials Science, (14 July 1993); doi: 10.1117/12.148038
Show Author Affiliations
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
K. A. Peterson, Stanford Univ. (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Satoshi Shimizu, Beckman Laser Institute and Medical Clinic (United States)
Chris W. Rella, Stanford Univ. (United States)
Andrew Gee Jeung, Stanford Univ. (United States)
Curtis F. Chapman, Beckman Laser Institute & Medical Clinic (United States)
Dana D. Dlott, Univ. of Illinois/Urbana-Champaign (United States)
Michael D. Fayer, Stanford Univ. (United States)
H. Alan Schwettman, Stanford Univ. (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 1854:
Free-Electron Laser Spectroscopy in Biology, Medicine, and Materials Science
H. Alan Schwettman, Editor(s)

© SPIE. Terms of Use
Back to Top