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Proceedings Paper

Helium-neon laser irradiation attenuates anoxia-induced degeneration of rabbit retinal ganglion cells
Author(s): Arieh S. Solomon; Avi Amir; Vered Lavie
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Paper Abstract

The effect of low-energy laser irradiation, known to delay axonal degeneration in mechanically traumatized nerves, was investigated in rabbit retinal ganglion cells damaged by temporary anoxia. Complete retinal vascular occlusion was induced in 39 rabbits by application of pressure to the cornea, with continuous monitoring under an operating microscope. The duration of occlusion was 15, 30 or 60 minutes. Starting immediately after the cessation of vascular occlusion, half of the rabbits in each group received transcorneal irradiation with a 35-mW helium-neon laser for 5 minutes daily on 10 consecutive days. The nonirradiated rabbits served as controls. Retinal ganglion cell viability was demonstrated by retrograde labeling of their axons with horseradish peroxidase, introduced subdurally into the optic nerve at a distance of 2 mm distal to the globe, 48 hours prior to sacrifice. For labeling intensity controls we used normal, not occluded, animals labeled with horseradish peroxidase by the same method. The animals were sacrificed 2, 4 or 8 weeks after occlusion. Labeling of retinal ganglion cells and their axons was observed in 100% of the normal control animals and in 85% of the irradiated rabbits. The results suggest that low-energy helium-neon laser irradiation attenuates the damage inflicted on the retinal ganglion cells as a result of anoxia.

Paper Details

Date Published: 14 July 1993
PDF: 7 pages
Proc. SPIE 1883, Low-Energy Laser Effects on Biological Systems, (14 July 1993); doi: 10.1117/12.148018
Show Author Affiliations
Arieh S. Solomon, Goldschleger Eye Research Institute (Israel)
Avi Amir, Goldschleger Eye Research Institute (Israel)
Vered Lavie, Weizmann Institute of Science (Israel)


Published in SPIE Proceedings Vol. 1883:
Low-Energy Laser Effects on Biological Systems
Michal Schwartz; Michael Belkin, Editor(s)

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