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Proceedings Paper

Helium-neon laser irradiation enhances DNA synthesis in a human neuroblastoma cell line
Author(s): Michael R. Condon; Frank Gump; Wen-hsien Wu
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Paper Abstract

To gain further insight into the mechanism of cell photostimulation by laser light ((lambda) equals 632.8 nm), DNA synthesis was measured in the human neuroblastoma cell line BE(2)-C. Cells were irradiated at high density to establish the characteristics of cellular energy into S- phase in response to laser stimulation. BE(2)-C cells after release from a quiescent, growth arrested state exhibited increased incorporation of isotope 12 hours after replating at subconfluent density in the presence of 2.5% fetal bovine serum (FBS) and [3H] thymidine. In contrast, cells replated under the same conditions, but stimulated with 15% FBS exhibited a time lag of approximately 16 hours in apparent DNA synthesis. These results were not corroborated by flow cytometry. Laser irradiation did not affect the fraction of cells entering S-phase. It therefore appears that the stimulatory effect of He-Ne laser irradiation on BE(2)-C cells is to enhance DNA synthesis while not altering the G1-S transition rate.

Paper Details

Date Published: 14 July 1993
PDF: 9 pages
Proc. SPIE 1883, Low-Energy Laser Effects on Biological Systems, (14 July 1993); doi: 10.1117/12.148015
Show Author Affiliations
Michael R. Condon, Veteran Affairs Medical Ctr. (United States)
Frank Gump, Veteran Affairs Medical Ctr. (United States)
Wen-hsien Wu, New Jersey Medical School (United States)

Published in SPIE Proceedings Vol. 1883:
Low-Energy Laser Effects on Biological Systems
Michal Schwartz; Michael Belkin, Editor(s)

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